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Proceedings Paper

Structured illumination 3D microscopy using adaptive lenses and multimode fibers
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Paper Abstract

Microscopic techniques with high spatial and temporal resolution are required for in vivo studying biological cells and tissues. Adaptive lenses exhibit strong potential for fast motion-free axial scanning. However, they also lead to a degradation of the achievable resolution because of aberrations. This hurdle can be overcome by digital optical technologies. We present a novel High-and-Low-frequency (HiLo) 3D-microscope using structured illumination and an adaptive lens. Uniform illumination is used to obtain optical sectioning for the high-frequency (Hi) components of the image, and nonuniform illumination is needed to obtain optical sectioning for the low-frequency (Lo) components of the image. Nonuniform illumination is provided by a multimode fiber. It ensures robustness against optical aberrations of the adaptive lens. The depth-of-field of our microscope can be adjusted a-posteriori by computational optics. It enables to create flexible scans, which compensate for irregular axial measurement positions. The adaptive HiLo 3D-microscope provides an axial scanning range of 1 mm with an axial resolution of about 4 microns and sub-micron lateral resolution over the full scanning range. In result, volumetric measurements with high temporal and spatial resolution are provided. Demonstration measurements of zebrafish embryos with reporter gene-driven fluorescence in the thyroid gland are presented.

Paper Details

Date Published: 26 June 2017
PDF: 6 pages
Proc. SPIE 10335, Digital Optical Technologies 2017, 1033519 (26 June 2017); doi: 10.1117/12.2270301
Show Author Affiliations
Jürgen Czarske, TU Dresden (Germany)
Katrin Philipp, TU Dresden (Germany)
Nektarios Koukourakis, TU Dresden (Germany)


Published in SPIE Proceedings Vol. 10335:
Digital Optical Technologies 2017
Bernard C. Kress; Peter Schelkens, Editor(s)

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