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Proceedings Paper

Raman imaging of molecular dynamics during cellular events
Author(s): Katsumasa Fujita
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Paper Abstract

To overcome the speed limitation in Raman imaging, we have developed a microscope system that detects Raman spectra from hundreds of points in a sample simultaneously. The sample was illuminated by a line-shaped focus, and Raman scattering from the illuminated positions was measured simultaneously by an imaging spectrophotometer. We applied the line-illumination technique to observe the dynamics of intracellular molecules during cellular events. We found that intracellular cytochrome c can be clearly imaged by resonant Raman scattering. We demonstrated label-free imaging of redistribution of cytochrome c during apoptosis and osteoblastic mineralization. We also proposed alkyne-tagged Raman imaging to observe small molecules in living cells. Due to its small size and the unique Raman band, alkyne can tag molecules without strong perturbation to molecular functions and with the capability to be detected separately from endogenous molecules.

Paper Details

Date Published: 12 July 2017
PDF: 4 pages
Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 1032409 (12 July 2017); doi: 10.1117/12.2270211
Show Author Affiliations
Katsumasa Fujita, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 10324:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
Jaebum Choo; Seung-Han Park, Editor(s)

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