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Proceedings Paper

Remote detection of buried explosives by fluorescent and bioluminescent microbial sensors (Conference Presentation)
Author(s): Shimshon Belkin; Sharon Yagur-Kroll; Cheinat Zohar; Zahi Rabinovitz; Amos Nussinovitch; Yossi Kabessa; Aharon J. Agranat

Paper Abstract

Current landmine detection methodologies are not much different in principle from those employed 75 years ago, in that they require actual presence in the minefield, with obvious risks to personnel and equipment. Other limitations include an extremely large ratio of false positives, as well as a very limited ability to detect non-metallic landmines. In this lecture a microbial-based solution for the remote detection of buried landmines described. The small size requirements, rapid responses and sensing versatility of bacterial bioreporters allow their integration into diverse types of devices, for laboratory as well as field applications. The relative ease by which molecular sensing and reporting elements can be fused together to generate dose-dependent quantifiable physical (luminescent, fluorescent, colorimetric, electrochemical) responses to pre-determined conditions allows the construction of diverse classes of sensors. Over the last two decades we and others have employed this principle to design and construct microbial bioreporter strains for the sensitive detection of (a) specific chemicals of environmental concern (heavy metals, halogenated organics etc.) or (b) their deleterious biological effects on living systems (such as toxicity or genotoxicity). In many of these cases, additional molecular manipulations beyond the initial sensor-reporter fusion may be highly beneficial for enhancing the performance of the engineered sensor systems. This presentation highlights several of the approaches we have adopted over the years to achieve this aim, while focusing on the application of live cell microbeads for the remote detection of buried landmines and other explosive devices.

Paper Details

Date Published: 9 June 2017
PDF: 1 pages
Proc. SPIE 10231, Optical Sensors 2017, 1023110 (9 June 2017); doi: 10.1117/12.2269691
Show Author Affiliations
Shimshon Belkin, The Hebrew Univ. of Jerusalem (Israel)
Sharon Yagur-Kroll, The Hebrew Univ. of Jerusalem (Israel)
Cheinat Zohar, The Hebrew Univ. of Jerusalem (Israel)
Zahi Rabinovitz, The Hebrew Univ. of Jerusalem (Israel)
Amos Nussinovitch, The Hebrew Univ. of Jerusalem (Israel)
Yossi Kabessa, The Hebrew Univ. of Jerusalem (Israel)
Aharon J. Agranat, The Hebrew Univ. of Jerusalem (Israel)

Published in SPIE Proceedings Vol. 10231:
Optical Sensors 2017
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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