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Proceedings Paper

Three-dimensional imaging of micro-specimen by optical scanning holography
Author(s): Jung-Ping Liu; Cheng-Hao Tsou
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Paper Abstract

Optical scanning holography (OSH) is a scanning-type digital holographic technique. In OSH, a heterodyne interference pattern is generated to raster scan the object. OSH can be operated in the incoherent mode and thus is able to record a fluorescence hologram. In addition, resolution of the OSH is proportional to the density of the interference pattern. Here we use a high-NA microscope objective to generate a dynamic Fresnel zone plate to record a hologram of micro-specimen. The achieved transverse resolution and longitudinal resolution are 0.78μm and 3.1μm, respectively.

Paper Details

Date Published: 18 April 2017
PDF: 2 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510D (18 April 2017); doi: 10.1117/12.2269503
Show Author Affiliations
Jung-Ping Liu, Feng Chia Univ. (Taiwan)
Cheng-Hao Tsou, Feng Chia Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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