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Proceedings Paper

Absorption contrast imaging beyond the diffraction limit with electron-beam excitation assisted optical microscope
Author(s): Wataru Inami; Masahiro Fukuta; Yoshimasa Kawata; Susumu Terakawa
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Paper Abstract

We demonstrated that the high spatial resolution absorption contrast imaging of the crystal of vitamin B9 having absorption at UV wavelengths. The absorption wavelength matches with the wavelength of the emission of the fluorescent thin film of an electron-beam excitation assisted (EXA) optical microscope. The fine crystal structure was imaged beyond the optical diffraction limit. The image contrast corresponded with the thickness of the crystal. The illumination light is absorbed with the vitamin B9 crystal and the intensity of the transmitted light depends on the thickness of the vitamin B9 crystal. The EXA optical microscope is useful for analysis of growth of a crystal, bioimaging, and so on.

Paper Details

Date Published: 18 April 2017
PDF: 3 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510G (18 April 2017); doi: 10.1117/12.2269437
Show Author Affiliations
Wataru Inami, Shizuoka Univ. (Japan)
Masahiro Fukuta, Shizuoka Univ. (Japan)
Yoshimasa Kawata, Shizuoka Univ. (Japan)
Susumu Terakawa, Tokoha Univ. (Japan)

Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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