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Proceedings Paper

Bayesian based fluorescence coded imaging using quantum dots
Author(s): Takahiro Nishimura; Hitoshi Kimura; Yusuke Ogura; Jun Tanida
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Paper Abstract

Single-molecule localization techniques are effective to resolve fluorescence images with higher resolution. To increase the frame rate, high-density positions of individual fluorescence emitters should be measured. We are studying a Bayesian-based localization method for measuring high density molecular positions with fluorescence coded images. In this paper, a scheme of several color quantum dots aligned with DNA nanostructures are considered. We confirmed that the proposed method could be applied to fluorescence images of quantum dots experimentally and that the positions of the aligned fluorescence emitters at intervals of 80 nm could be measured with little errors in numerical simulations.

Paper Details

Date Published: 18 April 2017
PDF: 2 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510Z (18 April 2017); doi: 10.1117/12.2269432
Show Author Affiliations
Takahiro Nishimura, Osaka Univ. (Japan)
Hitoshi Kimura, Osaka Univ. (Japan)
Yusuke Ogura, Osaka Univ. (Japan)
Jun Tanida, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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