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Proceedings Paper

Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging
Author(s): Guan-Yu Zhuo; Wei-Han Hung; Fu-Jen Kao
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Paper Abstract

The content of collagen is up to 30% existing in mammals. It supports the main component of connective tissues such as skin, ligament, and cartilage. Among various types of collagen, type-I collagen is of the most abundance and has been broadly studied due to the importance in bioscience. Second harmonic generation (SHG) microscopy is an effective tool used to study the collagen organization without labeling. In this study, we used circular polarization instead of linear polarization to retrieve three-dimensional (3D) molecular orientation of type-I collagen with only two cross polarized SHG images without acquiring an image stack of varying polarization.

Paper Details

Date Published: 18 April 2017
PDF: 3 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102511K (18 April 2017); doi: 10.1117/12.2269427
Show Author Affiliations
Guan-Yu Zhuo, National Sun Yat-sen Univ. (Taiwan)
Wei-Han Hung, National Sun Yat-sen Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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