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Proceedings Paper

Two-photon excitation microscopy with spatial light modulator
Author(s): Naoya Matsumoto; Alu Konno; Takashi Inoue; Haruyoshi Toyoda; Toshiyuki Miwa; Kazuhiro Nakamura; Shigetoshi Okazaki
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Paper Abstract

We attempted to observe deep regions in biological samples through two-photon excitation microscopy adopting a spatial light modulator (SLM). The SLM is used for correcting spherical aberration (SA) caused by the refractive-index mismatch between the immersion medium and sample. In the observation of fluorescent beads in transparent epoxy resin, the fluorescence intensity from a scan with SA correction was 50 times that from a scan without SA correction. After that, we observed blood vessels in a mouse brain, which became transparent with a clearing agent.

Paper Details

Date Published: 18 April 2017
PDF: 3 pages
Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025105 (18 April 2017); doi: 10.1117/12.2269406
Show Author Affiliations
Naoya Matsumoto, Hamamatsu Photonics K.K. (Japan)
Alu Konno, Hamamatsu Univ. School of Medicine (Japan)
Takashi Inoue, Hamamatsu Photonics K.K. (Japan)
Haruyoshi Toyoda, Hamamatsu Photonics K.K. (Japan)
Toshiyuki Miwa, Hamamatsu Photonics K.K. (Japan)
Kazuhiro Nakamura, Hamamatsu Photonics K.K. (Japan)
Shigetoshi Okazaki, Hamamatsu Univ. School of Medicine (Japan)

Published in SPIE Proceedings Vol. 10251:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji, Editor(s)

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