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Proceedings Paper

Bovine serum albumin nanoparticles loaded with Photosens photosensitizer for effective photodynamic therapy
Author(s): Vitaly Khanadeev; Boris Khlebtsov; Gopinath Packirisamy; Nikolai Khlebtsov
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Paper Abstract

Polymeric nanoparticles (NPs) are widely used for drug delivery applications due to high biodegradability, low toxicity and high loading capacity. The focus of this study is the development of photosensitizer Photosens (PS) loaded albumin NPs for efficient photodynamic therapy (PDT). To fabricate PS-loaded bovine serum albumin nanoparticles (BSA-PS NPs), we used a coacervation method with glutaraldehyde followed by passive loading of PS. Successful loading of PS was confirmed by appearance of characteristic peak in absorption spectrum which allows to determine the PS loading in BSA NPs. The synthesized BSA-PS NPs demonstrated low toxicity to HeLa cells at therapeutic concentrations of loaded PS. Compared to free PS solution, the synthesized BSA-PS NPs generated the singlet oxygen more effectively under laser irradiation at 660 nm. In addition, due to presence of various chemical groups on the surface of BSA-PS NPs, they are capable to adsorb on cell surface and accumulate in cells due to cellular uptake mechanisms. Owing to combination of PD and cell uptake advantages, BSA-PS NPs demonstrated higher efficacy of photodynamic damage to cancer cells as compared to free PS at equivalent concentrations. These results suggest that non-targeted BSA-PS NPs with high PD activity and low-fabrication costs of are promising candidates for transfer to PD clinic treatments.

Paper Details

Date Published: 24 March 2017
PDF: 7 pages
Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360P (24 March 2017); doi: 10.1117/12.2269159
Show Author Affiliations
Vitaly Khanadeev, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Boris Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Saratov National Research State Univ. (Russian Federation)
Gopinath Packirisamy, Indian Institute of Technology Roorkee (India)
Nikolai Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Saratov National Research State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10336:
Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII
Valery V. Tuchin; Elina A. Genina; Dmitry E. Postnov; Vladimir L. Derbov, Editor(s)

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