Share Email Print

Proceedings Paper

Plasmonic nanochip for SERS chemical and biomedical sensing
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The development of rapid, easy-to-use and highly sensitive DNA detection methods has received increasing interest for medical diagnostics and research purposes. Our laboratory has developed several chip-based DNA biosensors including molecular sentinel-on-chip (MSC), multiplex MSC, and inverse molecular sentinel-on-chip (iMS-on-Chip). These sensors use surface-enhanced Raman scattering (SERS) plasmonic chips, functionalized with DNA probes for single-step DNA detection. The sensing mechanisms is based on the hybridization of target sequences and DNA probes, resulting in a displacement of a SERS reporter from the chip surface. This distance increase results in change in SERS signal intensity from the reporter, thus indicating the capture, and therefore the presence, of the target nucleic acid sequence. The nucleic acid probes and the SERS chip, which compose the sensing platform, were designed for single-step DNA detection. The target sequences are detected by delivery of a sample solutions on a functionalized chip and characterization of the SERS signals, after 1 - 2 hr incubation. These techniques avoid labeling of the target sequence or washing to remove unreacted components, making them easy-to-use and cost effective. The use of SERS chip for medical diagnostics was demonstrated by detecting genetic biomarkers for respiratory viral infection and the DNA of dengue virus 4.

Paper Details

Date Published: 3 May 2017
PDF: 6 pages
Proc. SPIE 10215, Advanced Environmental, Chemical, and Biological Sensing Technologies XIV, 102150G (3 May 2017); doi: 10.1117/12.2267759
Show Author Affiliations
Hoan T. Ngo, Duke Univ. (United States)
Pietro Strobbia, Duke Univ. (United States)
Hsin-Neng Wang, Duke Univ. (United States)
Bridget M. Crawford, Duke Univ. (United States)
Andrew M. Fales, Duke Univ. (United States)
Tuan Vo-Dinh, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 10215:
Advanced Environmental, Chemical, and Biological Sensing Technologies XIV
Tuan Vo-Dinh; Robert A. Lieberman, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?