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Proceedings Paper

Nanosensors for nucleic acid targets detection using SERS
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Paper Abstract

Single cell analysis can aid the study of molecular events responsible for cellular functions and unveil their connections to the biological functions of an organism. Biosensors based on surface enhanced Raman spectroscopy (SERS) can be used to this end and offer several advantages over other sensing platforms, such as sensitivity and multiplexed capabilities, among others. While SERS nanosensors/nanoparticles have been used for analysis in single cells, the delivery of such biosensors relies on cellular uptake, which requires long incubation time and has different efficiencies among cell lines. Nanosensors based on tapered optical fibers, instead, can be inserted in single cells and detect target molecules in subcellular compartment. The combination of these sensing devices with the transduction mechanism of nucleic acid based nanoprobes (i.e. inverse molecular sentinels) will permit the more direct detection of nucleic acids within single cells. This paper presents the development of tapered fiber-based biosensors for the detection of nucleic acid biomarkers in plant cells. The use of inverse molecular sentinels in plant cell was demonstrated. Sensors based on tapered fibers were fabricated and used to measure SERS from a single cell.

Paper Details

Date Published: 3 May 2017
PDF: 6 pages
Proc. SPIE 10215, Advanced Environmental, Chemical, and Biological Sensing Technologies XIV, 1021508 (3 May 2017); doi: 10.1117/12.2267757
Show Author Affiliations
Pietro Strobbia, Duke Univ. (United States)
Bridget M. Crawford, Duke Univ. (United States)
Hsin-Neng Wang, Duke Univ. (United States)
Tuan Vo-Dinh, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 10215:
Advanced Environmental, Chemical, and Biological Sensing Technologies XIV
Tuan Vo-Dinh; Robert A. Lieberman, Editor(s)

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