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Proceedings Paper

Specific detection of very low concentrations of DNA oligonucleotides with DNA-coated long-period grating biosensor
Author(s): Karolina H. Czarnecka; Magdalena Dominik; Marta Janczuk-Richter; Joanna Niedziółka-Jönsson; Wojtek J. Bock; Mateusz Śmietana
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Paper Abstract

Label-free biosensing using optical long-period gratings (LPGs) induced in optical fiber and coated with biological compound enables for detection and monitoring the kinetics of reactions taking places on the sensor’s surface. The labelfree detection effect for the LPG biosensor working near the dispersion turning point (DTP) of higher order cladding modes can be observed as resonance wavelength shift induced by binding of biomolecules to fiber surface. In this study we analyzed shift of the resonance wavelength for LPG after its functionalization with DNA layer and during DNA hybridization. It has seen found that the LPG is able to be functionalized with DNA oligonucleotide, acting as a probe and after the functionalization can selectively bind specific complementary DNA oligonucleotides in a very low concentration. DNA-coated LPG can be used as a probe capturing specific DNA sequences with wide ranges of concentrations from 0.1 to 10 pM. Hybridization of the DNA on the fiber surface has also been verified with Midorii Green florescent marker.

Paper Details

Date Published: 23 April 2017
PDF: 4 pages
Proc. SPIE 10323, 25th International Conference on Optical Fiber Sensors, 1032313 (23 April 2017); doi: 10.1117/12.2267411
Show Author Affiliations
Karolina H. Czarnecka, Warsaw Univ. of Technology (Poland)
Medical Univ. of Lodz (Poland)
Magdalena Dominik, Warsaw Univ. of Technology (Poland)
Marta Janczuk-Richter, Institute of Physical Chemistry (Poland)
Joanna Niedziółka-Jönsson, Institute of Physical Chemistry (Poland)
Wojtek J. Bock, Univ. du Québec en Outaouais (Canada)
Mateusz Śmietana, Warsaw Univ. of Technology (Poland)

Published in SPIE Proceedings Vol. 10323:
25th International Conference on Optical Fiber Sensors
Youngjoo Chung; Wei Jin; Byoungho Lee; John Canning; Kentaro Nakamura; Libo Yuan, Editor(s)

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