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Proceedings Paper

Preparation of Mach-Zehnder interferometric photonic biosensors by inkjet printing technology
Author(s): Florian Strasser; Eva Melnik; Paul Muellner; Pilar Jiménez-Meneses; Magdalena Nechvile; Guenther Koppitsch; Peter Lieberzeit; Michael Laemmerhofer; Rudolf Heer; Rainer Hainberger
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Paper Abstract

Inkjet printing is a versatile method to apply surface modification procedures in a spatially controlled, cost-effective and mass-fabrication compatible manner. Utilizing this technology, we investigate two different approaches for functionalizing label-free optical waveguide based biosensors: a) surface modification with amine-based functional polymers (biotin-modified polyethylenimine (PEI-B)) employing active ester chemistry and b) modification with dextran based hydrogel thin films employing photoactive benzophenone crosslinker moieties. Whereas the modification with PEI-B ensures high receptor density at the surface, the hydrogel films can serve both as a voluminous matrix binding matrix and as a semipermeable separation layer between the sensor surface and the sample. We use the two surface modification strategies both individually and in combination for binding studies towards the detection of the protein inflammation biomarker, C-reactive protein (CRP). For the specific detection of CRP, we compare two kinds of capture molecules, namely biotinylated antibodies and biotinylated CRP-specific DNA based aptamers. Both kinds of capture molecules were immobilized on the PEI-B by means of streptavidin-biotin affinity binding. As transducer, we use an integrated four-channel silicon nitride (Si3N4) waveguide based Mach-Zehnder interferometric (MZI) photonic sensing platform operating at a wavelength of 850nm (TM-mode).

Paper Details

Date Published: 16 May 2017
PDF: 7 pages
Proc. SPIE 10231, Optical Sensors 2017, 102312H (16 May 2017); doi: 10.1117/12.2267156
Show Author Affiliations
Florian Strasser, AIT Austrian Institute of Technology GmbH (Austria)
Eva Melnik, AIT Austrian Institute of Technology GmbH (Austria)
Paul Muellner, AIT Austrian Institute of Technology GmbH (Austria)
Pilar Jiménez-Meneses, Univ. Politècnica de València (Spain)
Magdalena Nechvile, AIT Austrian Institute of Technology GmbH (Austria)
Univ. of Vienna (Austria)
Guenther Koppitsch, ams AG (Austria)
Peter Lieberzeit, Univ. Vienna (Austria)
Michael Laemmerhofer, Eberhard Karls Univ. Tübingen (Germany)
Rudolf Heer, AIT Austrian Institute of Technology GmbH (Austria)
Rainer Hainberger, AIT Austrian Institute of Technology GmbH (Austria)

Published in SPIE Proceedings Vol. 10231:
Optical Sensors 2017
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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