The transport of intensity equation (TIE) is a powerful tool for direct quantitative phase retrieval in microscopy imaging. However, there may be some problems when dealing with the boundary condition of the TIE. The previous work introduces a hard-edged aperture to the camera port of the traditional bright field microscope to generate the boundary signal for the TIE solver. Under this Neumann boundary condition, we can obtain the quantitative phase without any assumption or prior knowledge about the test object and the setup. In this paper, we will demonstrate the effectiveness of this method based on some experiments in practice. The micro lens array will be used for the comparison of two TIE solvers results based on introducing the aperture or not and this accurate quantitative phase imaging technique allows measuring cell dry mass which is used in biology to follow cell cycle, to investigate cell metabolism, or to address effects of drugs.

Date Published: 10 February 2017
PDF: 5 pages
Proc. SPIE 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016), 102501A (10 February 2017); doi: 10.1117/12.2266907

Published in SPIE Proceedings Vol. 10250:
International Conference on Optical and Photonics Engineering (icOPEN 2016)
Anand Krishna Asundi; Xiyan Huang; Yi Xie, Editor(s)