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Proceedings Paper

Implementation of stimulated Raman scattering microscopy for single cell analysis
Author(s): Annalisa D'Arco; Maria Antonietta Ferrara; Maurizio Indolfi; Vitaliano Tufano; Luigi Sirleto
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Paper Abstract

In this work, we present successfully realization of a nonlinear microscope, not purchasable in commerce, based on stimulated Raman scattering. It is obtained by the integration of a femtosecond SRS spectroscopic setup with an inverted research microscope equipped with a scanning unit. Taking account of strength of vibrational contrast of SRS, it provides label-free imaging of single cell analysis. Validation tests on images of polystyrene beads are reported to demonstrate the feasibility of the approach. In order to test the microscope on biological structures, we report and discuss the label-free images of lipid droplets inside fixed adipocyte cells.

Paper Details

Date Published: 16 May 2017
PDF: 8 pages
Proc. SPIE 10228, Nonlinear Optics and Applications X, 102280S (16 May 2017); doi: 10.1117/12.2266776
Show Author Affiliations
Annalisa D'Arco, Consiglio Nazionale delle Ricerche (Italy)
Maria Antonietta Ferrara, Consiglio Nazionale delle Ricerche (Italy)
Maurizio Indolfi, Consiglio Nazionale delle Ricerche (Italy)
Vitaliano Tufano, Consiglio Nazionale delle Ricerche (Italy)
Luigi Sirleto, Consiglio Nazionale delle Ricerche (Italy)

Published in SPIE Proceedings Vol. 10228:
Nonlinear Optics and Applications X
Mario Bertolotti; Joseph W. Haus; Alexei M. Zheltikov, Editor(s)

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