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Proceedings Paper

Quantitative image analysis for investigating cell-matrix interactions
Author(s): Brian Burkel; Jacob Notbohm
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Paper Abstract

The extracellular matrix provides both chemical and physical cues that control cellular processes such as migration, division, differentiation, and cancer progression. Cells can mechanically alter the matrix by applying forces that result in matrix displacements, which in turn may localize to form dense bands along which cells may migrate. To quantify the displacements, we use confocal microscopy and fluorescent labeling to acquire high-contrast images of the fibrous material. Using a technique for quantitative image analysis called digital volume correlation, we then compute the matrix displacements. Our experimental technology offers a means to quantify matrix mechanics and cell-matrix interactions. We are now using these experimental tools to modulate mechanical properties of the matrix to study cell contraction and migration.

Paper Details

Date Published: 12 July 2017
PDF: 5 pages
Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 1032403 (12 July 2017); doi: 10.1117/12.2266595
Show Author Affiliations
Brian Burkel, Univ. of Wisconsin-Madison (United States)
Jacob Notbohm, Univ. of Wisconsin-Madison (United States)

Published in SPIE Proceedings Vol. 10324:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
Jaebum Choo; Seung-Han Park, Editor(s)

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