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Proceedings Paper

Double resonance long period fiber grating for detection of E. coli in trace concentration by choosing a proper bacteriophage
Author(s): Y. Chiniforooshan; A. Celebanska; M. Janik; P. Mikulic; F. Haddad; J. Perreault; W. J. Bock
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Paper Abstract

There is a critical need of a fast, specific and reliable assay for biological species. To address this need, long period fiber gratings (LPFG) among other fiber optic sensors can be used because of their high sensitivity to changes in surrounding medium. In this work we fabricated and used two over-etched LPFGs. One of them was covered with T4 Phage and the other was covered with MS2 phage that both specifically bind with Escherichia coli (E. coli) bacteria. This bacterium is a major cause of the food contaminations and outbreaks. We showed achieving a highest sensitivity region of the LPFG and the way to fine tune to that region by over-etching the grating. Finally, using the highly sensitive LPFG platform we could detect E. coli at concentrations as low as 100 colony forming units (CFU), by covering the LPFG with an optimized bio-functionalization of the fiber surface with MS2 bacteriophage.

Paper Details

Date Published: 23 April 2017
PDF: 4 pages
Proc. SPIE 10323, 25th International Conference on Optical Fiber Sensors, 103234X (23 April 2017);
Show Author Affiliations
Y. Chiniforooshan, Univ. du Québec en Outaouais (Canada)
A. Celebanska, Univ. du Québec en Outaouais (Canada)
M. Janik, Univ. du Québec en Outaouais (Canada)
P. Mikulic, Univ. du Québec en Outaouais (Canada)
F. Haddad, Ctr. INRS-Institut Armand-Frappier (Canada)
J. Perreault, Ctr. INRS-Institut Armand-Frappier (Canada)
W. J. Bock, Univ. du Québec en Outaouais (Canada)

Published in SPIE Proceedings Vol. 10323:
25th International Conference on Optical Fiber Sensors
Youngjoo Chung; Wei Jin; Byoungho Lee; John Canning; Kentaro Nakamura; Libo Yuan, Editor(s)

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