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Proceedings Paper

Development of a lab-on-chip electrochemical immunosensor for detection of Polycyclic Aromatic Hydrocarbons (PAH) in environmental water
Author(s): Shifa Felemban; Patricia Vazquez; Jan Dehnert; Vadim Goridko; Maria Tijero; Eric Moore
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Paper Abstract

The work described in this manuscript focuses on how the integration of immunoassay techniques in combination with electrochemical detection can provide a portable and very accurate solution for detection of water pollutants that are detrimental for human health. In particular, we focus our work on the quantification of polycyclic aromatic hydrocarbons (PAHs) in polluted water. Our integrative approach facilitates a real-time detection of this family of organic compounds, by reducing the time of analysis to less than one hour. Additionally, the use of a lab-on-a-chip platform delivers a portable solution that could be used in situ.

Optimization of a displacement assay that investigates the presence and concentration of Benzo[a]pyrene in water, allows with the miniaturization of the standard ELISA format into a highly accurate system that provides fast results. The limits of detection obtained are comparable to those of available state-of-the art tools, and achieve the values set by European Drinking Water Directive, 0.10ng/l, as the limit for PAHs in drinking water.

Paper Details

Date Published: 13 June 2017
PDF: 11 pages
Proc. SPIE 10246, Smart Sensors, Actuators, and MEMS VIII, 102460B (13 June 2017); doi: 10.1117/12.2265011
Show Author Affiliations
Shifa Felemban, Tyndall National Institute (Ireland)
Patricia Vazquez, Tyndall National Institute (Ireland)
Jan Dehnert, Hahn-Schickard (Germany)
Vadim Goridko, Hahn-Schickard (Germany)
Maria Tijero, IK4-IKERLAN (Spain)
Eric Moore, Univ. College Cork (Ireland)

Published in SPIE Proceedings Vol. 10246:
Smart Sensors, Actuators, and MEMS VIII
Luis Fonseca; Mika Prunnila; Erwin Peiner, Editor(s)

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