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Proceedings Paper

Infrared spectroscopy as a screening technique for colitis
Author(s): Jitto Titus; Hemendra Ghimire; Emilie Viennois; Didier Merlin; A. G. Unil Perera
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Paper Abstract

There remains a great need for diagnosis of inflammatory bowel disease (IBD), for which the current technique, colonoscopy, is not cost-effective and presents a non-negligible risk for complications. Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy is a new screening technique to evaluate colitis. Comparing infrared spectra of sera to study the differences between them can prove challenging due to the complexity of its biological constituents giving rise to a plethora of vibrational modes. Overcoming these inherent infrared spectral analysis difficulties involving highly overlapping absorbance peaks and the analysis of the data by curve fitting to improve the resolution is discussed. The proposed technique uses colitic and normal wild type mice dried serum to obtain ATR/FTIR spectra to effectively differentiate colitic mice from normal mice. Using this method, Amide I group frequency (specifically, alpha helix to beta sheet ratio of the protein secondary structure) was identified as disease associated spectral signature in addition to the previously reported glucose and mannose signatures in sera of chronic and acute mice models of colitis. Hence, this technique will be able to identify changes in the sera due to various diseases.

Paper Details

Date Published: 30 May 2017
PDF: 11 pages
Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 102470C (30 May 2017); doi: 10.1117/12.2264462
Show Author Affiliations
Jitto Titus, Georgia State Univ. (United States)
Hemendra Ghimire, Georgia State Univ. (United States)
Emilie Viennois, Georgia State Univ. (United States)
Didier Merlin, Georgia State Univ. (United States)
A. G. Unil Perera, Georgia State Univ. (United States)

Published in SPIE Proceedings Vol. 10247:
Bio-MEMS and Medical Microdevices III
Sander van den Driesche; Ioanna Giouroudi; Manuel Delgado-Restituto, Editor(s)

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