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Proceedings Paper

Analysis of off-axis incoherent digital holographic microscopy
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Paper Abstract

Off-axis incoherent digital holography that enables single-shot three-dimensional (3D) distribution is introduced in the paper. Conventional fluorescence microscopy images 3D fields by sectioning, this prevents instant imaging of fast reactions of living cells. In order to realize digital holography from incoherent light, we adapted common path configuration to achieve the best temporal coherence. And by introducing gratings, we shifted the direction of each light to achieve off-axis interference. Simulations and preliminary experiments using LED light have confirmed the results. We expect to use this method to realize 3D phase imaging and fluorescent imaging at the same time from the same biological sample.

Paper Details

Date Published: 10 May 2017
PDF: 6 pages
Proc. SPIE 10219, Three-Dimensional Imaging, Visualization, and Display 2017, 102190U (10 May 2017); doi: 10.1117/12.2264217
Show Author Affiliations
Xiangyu Quan, Kobe Univ. (Japan)
Osamu Matoba, Kobe Univ. (Japan)
Yasuhiro Awatsuji, Kyoto Institute of Technology (Japan)

Published in SPIE Proceedings Vol. 10219:
Three-Dimensional Imaging, Visualization, and Display 2017
Bahram Javidi; Jung-Young Son; Osamu Matoba, Editor(s)

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