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Proceedings Paper

Inkjet-printed selective microfluidic biosensor using CNTs functionalized by cytochrome P450 enzyme
Author(s): Matic Krivec; Raimund Leitner; Florian Überall; Johannes Hochleitner
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Paper Abstract

An additive manufacturing concept, consisting of 3D photopolymer printing and Ag nanoparticle printing, was investigated for the construction of a microfluidic biosensor based on immobilized cytochrome P450 enzyme. An acylate-type microfluidic chamber composed of two parts, i.e. chamber-housing and chamber-lid was printed with a polyjet 3D printer. A 3-electrode sensor structure was inkjet-printed on the lid using a combination of Ag and graphene printing. The working electrode was covered with carbon nanotubes by drop-casting and immobilized with cytochrome P450 2D6 enzyme. The microfluidic sensor shows a significant response to a test xenobiotic, i.e. dextromethorphan; the cyclic voltammetrical measurements show a corresponding oxidation peak at 0.4 V with around 5 μM detection limit.

Paper Details

Date Published: 30 May 2017
PDF: 7 pages
Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 1024707 (30 May 2017); doi: 10.1117/12.2264095
Show Author Affiliations
Matic Krivec, CTR Carinthian Tech Research AG (Austria)
Raimund Leitner, CTR Carinthian Tech Research AG (Austria)
Florian Überall, Medizinische Univ. Innsbruck (Austria)
Johannes Hochleitner, Medizinische Univ. Innsbruck (Austria)

Published in SPIE Proceedings Vol. 10247:
Bio-MEMS and Medical Microdevices III
Sander van den Driesche; Ioanna Giouroudi; Manuel Delgado-Restituto, Editor(s)

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