An additive manufacturing concept, consisting of 3D photopolymer printing and Ag nanoparticle printing, was investigated for the construction of a microfluidic biosensor based on immobilized cytochrome P450 enzyme. An acylate-type microfluidic chamber composed of two parts, i.e. chamber-housing and chamber-lid was printed with a polyjet 3D printer. A 3-electrode sensor structure was inkjet-printed on the lid using a combination of Ag and graphene printing. The working electrode was covered with carbon nanotubes by drop-casting and immobilized with cytochrome P450 2D6 enzyme. The microfluidic sensor shows a significant response to a test xenobiotic, i.e. dextromethorphan; the cyclic voltammetrical measurements show a corresponding oxidation peak at 0.4 V with around 5 μM detection limit.

Date Published: 30 May 2017
PDF: 7 pages
Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 1024707 (30 May 2017); doi: 10.1117/12.2264095

Published in SPIE Proceedings Vol. 10247:
Bio-MEMS and Medical Microdevices III
Sander van den Driesche; Ioanna Giouroudi; Manuel Delgado-Restituto, Editor(s)