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Proceedings Paper

Supramolecular delivery of photoactivatable fluorophores in developing embryos
Author(s): Yang Zhang; Sicheng Tang; Lorenzo Sansalone; Ek Raj Thapaliya; James D. Baker; Françisco M. Raymo
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Paper Abstract

The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.

Paper Details

Date Published: 22 February 2017
PDF: 6 pages
Proc. SPIE 10078, Colloidal Nanoparticles for Biomedical Applications XII, 100781A (22 February 2017); doi: 10.1117/12.2261721
Show Author Affiliations
Yang Zhang, Univ. of Miami (United States)
Sicheng Tang, Univ. of Miami (United States)
Lorenzo Sansalone, Univ. of Miami (United States)
Ek Raj Thapaliya, Univ. of Miami (United States)
James D. Baker, Univ. of Miami (United States)
Françisco M. Raymo, Univ. of Miami (United States)

Published in SPIE Proceedings Vol. 10078:
Colloidal Nanoparticles for Biomedical Applications XII
Marek Osiński; Wolfgang J. Parak; Xing-Jie Liang, Editor(s)

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