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Proceedings Paper

A portable fluorescent sensing system using multiple LEDs
Author(s): Young-Ho Shin; Jonathan Z. Barnett; M. Teresa Gutierrez-Wing; Kelly A. Rusch; Jin-Woo Choi
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Paper Abstract

This paper presents a portable fluorescent sensing system that utilizes different light emitting diode (LED) excitation lights for multiple target detection. In order to identify different analytes, three different wavelengths (385 nm, 448 nm, and 590 nm) of excitation light emitting diodes were used to selectively stimulate the target analytes. A highly sensitive silicon photomultiplier (SiPM) was used to detect corresponding fluorescent signals from each analyte. Based on the unique fluorescent response of each analyte, it is possible to simultaneously differentiate one analyte from the other in a mixture of target analytes. A portable system was designed and fabricated consisting of a display module, battery, data storage card, and sample loading tray into a compact 3D-printed jig. The portable sensor system was demonstrated for quantification and differentiation of microalgae (Chlorella vulgaris) and cyanobacteria (Spirulina) by measuring fluorescent responses of chlorophyll a in microalgae and phycocyanin in cyanobacteria. Obtained results suggest that the developed portable sensor system could be used as a generic fluorescence sensor platform for on-site detection of multiple analytes of interest.

Paper Details

Date Published: 28 February 2017
PDF: 7 pages
Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610M (28 February 2017); doi: 10.1117/12.2261292
Show Author Affiliations
Young-Ho Shin, Louisiana State Univ. (United States)
Jonathan Z. Barnett, Louisiana State Univ. (United States)
M. Teresa Gutierrez-Wing, Louisiana State Univ. (United States)
Kelly A. Rusch, Lousiana State Univ. (United States)
North Dakota State Univ. (United States)
Jin-Woo Choi, Louisiana State Univ. (United States)

Published in SPIE Proceedings Vol. 10061:
Microfluidics, BioMEMS, and Medical Microsystems XV
Bonnie L. Gray; Holger Becker, Editor(s)

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