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Proceedings Paper

Single cell manipulation utilizing femtosecond laser-induced shock and stress waves
Author(s): Yoichiroh Hosokawa
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Paper Abstract

When an intense femtosecond laser pulse is focused into a culture medium through an objective lens, an impulsive force is loaded on the cells with generations of the shock and stress waves at the laser focal point. The shock and stress waves were acted to single cells in the vicinity of the laser focal point as an impulsive force. We have applied the impulsive force to manipulate single cells. As the transient intensity of the impulsive force is over 1000 times stronger than the force due to optical tweezers, drastic single manipulation which is difficult by the optical tweezers can be realized. The generation process of the impulsive force and behavior of animal cell after loading the impulsive force were reviewed, and then our original quantification method of the impulsive force utilizing atomic force microscope (AFM) was introduced with its applications for evaluating adhesions between animal cells and between sub-organelles in plant cell.

Paper Details

Date Published: 17 February 2017
PDF: 6 pages
Proc. SPIE 10094, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII, 100940A (17 February 2017); doi: 10.1117/12.2260794
Show Author Affiliations
Yoichiroh Hosokawa, Nara Institute of Science and Technology (Japan)

Published in SPIE Proceedings Vol. 10094:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Roberto Osellame, Editor(s)

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