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Proceedings Paper

Multi-scale silica structures for improved point of care detection
Author(s): Sophia Lin; Lancy Lin; Eunbyul Cho; Gaston A. O. Pezzani; Michelle Khine
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Paper Abstract

The need for sensitive, portable diagnostic tests at the point of care persists. We report on a simple method to obtain improved detection of biomolecules by a two-fold mechanism. Silica (SiO2) is coated on pre-stressed thermoplastic shrink-wrap film. When the film retracts, the resulting micro- and nanostructures yield far-field fluorescence signal enhancements over their planar or wrinkled counterparts. Because the film shrinks by 95% in surface area, there is also a 20x concentration effect. The SiO2 structured substrate is therefore used for improved detection of labeled proteins and DNA hybridization via both fluorescent and bright field. Through optical characterization studies, we attribute the fluorescence signal enhancements of ~100x to increased surface density and light scattering from the rough SiO2 structures. Combining with our open channel self-wicking microfluidics, we can achieve extremely low cost yet sensitive point of care diagnostics.

Paper Details

Date Published: 3 March 2017
PDF: 4 pages
Proc. SPIE 10055, Optics and Biophotonics in Low-Resource Settings III, 100550S (3 March 2017); doi: 10.1117/12.2258655
Show Author Affiliations
Sophia Lin, Univ. of California, Irvine (United States)
Lancy Lin, Univ. of California, Irvine (United States)
Eunbyul Cho, Univ. of California, Irvine (United States)
Gaston A. O. Pezzani, Univ. of California, Irvine (United States)
Michelle Khine, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 10055:
Optics and Biophotonics in Low-Resource Settings III
David Levitz; Aydogan Ozcan; David Erickson, Editor(s)

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