Share Email Print

Proceedings Paper • Open Access

Label-free tomographic reconstruction of optically thick structures using GLIM (Conference Presentation)
Author(s): Mikhail E. Kandel; Ghazal N. Kouzehgarani; Tan H. Ngyuen; Martha U. Gillette; Gabriel Popescu

Paper Abstract

Although the contrast generated in transmitted light microscopy is due to the elastic scattering of light, multiple scattering scrambles the image and reduces overall visibility. To image both thin and thick samples, we turn to gradient light interference microscopy (GLIM) to simultaneously measure morphological parameters such as cell mass, volume, and surfaces as they change through time. Because GLIM combines multiple intensity images corresponding to controlled phase offsets between laterally sheared beams, incoherent contributions from multiple scattering are implicitly cancelled during the phase reconstruction procedure. As the interfering beams traverse near identical paths, they remain comparable in power and interfere with optimal contrast. This key property lets us obtain tomographic parameters from wide field z-scans after simple numerical processing. Here we show our results on reconstructing tomograms of bovine embryos, characterizing the time-lapse growth of HeLa cells in 3D, and preliminary results on imaging much larger specimen such as brain slices.

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10074, Quantitative Phase Imaging III, 1007403 (24 April 2017); doi: 10.1117/12.2257046
Show Author Affiliations
Mikhail E. Kandel, Univ. of Illinois at Urbana-Champaign (United States)
Ghazal N. Kouzehgarani, Univ. of Illinois at Urbana-Champaign (United States)
Tan H. Ngyuen, Univ. of Illinois at Urbana-Champaign (United States)
Martha U. Gillette, Univ. of Illinois at Urbana-Champaign (United States)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 10074:
Quantitative Phase Imaging III
Gabriel Popescu; YongKeun Park, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?