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Proceedings Paper

Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy
Author(s): L. Gardini; F. S. Pavone; M. Capitanio
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Paper Abstract

Here we present development of optical techniques for the study of single processive myosin motors based on the combination of high-speed optical tweezers force spectroscopy and single molecule fluorescence imaging. Ultrafast force-clamp spectroscopy1 is applied to study the dependence of single chemo-mechanical steps of processive myosin motors on the applied load. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy)2, 3 is applied to in vitro motility assay to measure parameters such as the runlength, velocity and step size of single myosin V motors, labeled with Quantum Dots, under unloaded conditions.

Paper Details

Date Published: 21 February 2017
PDF: 6 pages
Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 1007111 (21 February 2017);
Show Author Affiliations
L. Gardini, Lab. Europeo di Spettroscopie Non-Lineari (Italy)
F. S. Pavone, Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. of Florence (Italy)
Instituto Nazionale di Ottica (Italy)
M. Capitanio, Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 10071:
Single Molecule Spectroscopy and Superresolution Imaging X
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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