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Proceedings Paper

Comparison of continuous versus pulsed photodynamic antimicrobial therapy for inhibition of fungal keratitis isolates in vitro (Conference Presentation)
Author(s): Nicholas Nolan; Heather A. Durkee; Mariela C. Aguilar; Alejandro Arboleda; Nidhi Relhan; Anna Martinez; Cornelis Rowaan; Alex Gonzalez; Karam A. Alawa; Guillermo Amescua; Harry W. Flynn Jr.; Darlene Miller; Jean-Marie A. Parel

Paper Abstract

Fungal keratitis can lead to pain and impaired vision. Current treatment options include antifungal agents and therapeutic penetrating keratoplasty. An emerging option for the management of keratitis is photodynamic antimicrobial therapy (PDAT) which uses a photosensitizer rose bengal activated with green light. Utilizing a pulsed irradiation, rather than the standard continuous irradiation may have a similar antimicrobial effect with less total energy. This study is to compare pulsed and continuous rose bengal mediated PDAT for inhibition of six fungal isolates on agar plates: Fusarium solani, Fusarium keratoplasticum, Aspergillus fumigatus, Candida albicans, Paecilomyces variotti, and Pseudoallescheria boydii. Isolates were mixed with 0.1% rose bengal and exposed to three irradiation conditions: (1) 30-minute continuous (10.8J/cm2), (2) 15-minute continuous (5.4J/cm2), (3) 30-minute pulsed (5.4J/cm2). Plates were photographed at 72 hours and analyzed with custom software. At 72 hours, 30-minute continuous rose bengal mediated PDAT inhibited all six fungal species. Fungal inhibition was analogous between 30-minute continuous and 30-minute pulsed test groups, with the exception of A. fumigatus. The 15-minute continuous irradiation was less effective when compared to both 30-minute continuous and 30-minute pulsed groups. These in vitro results demonstrate the potential strength of pulsed rose bengal mediated PDAT as an adjunct treatment modality for fungal keratitis.

Paper Details

Date Published: 16 May 2017
PDF: 1 pages
Proc. SPIE 10045, Ophthalmic Technologies XXVII, 1004506 (16 May 2017); doi: 10.1117/12.2256183
Show Author Affiliations
Nicholas Nolan, Ophthalmic Biophysics Ctr. (United States)
Heather A. Durkee, Ophthalmic Biophysics Ctr. (United States)
Mariela C. Aguilar, Ophthalmic Biophysics Ctr. (United States)
Alejandro Arboleda, Ophthalmic Biophysics Ctr. (United States)
Nidhi Relhan, Ophthalmic Biophysics Ctr. (United States)
Anna Martinez, Ophthalmic Biophysics Ctr. (United States)
Cornelis Rowaan, Ophthalmic Biophysics Ctr. (United States)
Alex Gonzalez, Ophthalmic Biophysics Ctr. (United States)
Karam A. Alawa, Ophthalmic Biophysics Ctr. (United States)
Guillermo Amescua, Anne Bates Leach Eye Hospital (United States)
Harry W. Flynn Jr., Anne Bates Leach Eye Hospital (United States)
Darlene Miller, Ocular Microbiology Lab., Bascom Palmer Eye Institute (United States)
Jean-Marie A. Parel, Ophthalmic Biophysics Ctr. (United States)

Published in SPIE Proceedings Vol. 10045:
Ophthalmic Technologies XXVII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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