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Proceedings Paper

Open-air multispectral fluorescence-guided surgery platform for intraoperative detection of malignant tissue under ambient lighting conditions
Author(s): Ali Behrooz; Kristine O. Vasquez; Peter Waterman; Jeff Meganck; Jeffrey D. Peterson; Peter Miller; Joshua Kempner
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Paper Abstract

Intraoperative resection of tumors currently relies upon the surgeon’s ability to visually locate and palpate tumor nodules. Undetected residual malignant tissue often results in the need for additional treatment or surgical intervention. The Solaris platform is a multispectral open-air fluorescence imaging system designed for translational fluorescence-guided surgery. Solaris supports video-rate imaging in four fixed fluorescence channels ranging from visible to near infrared, and a multispectral channel equipped with a liquid crystal tunable filter (LCTF) for multispectral image acquisition (520-620 nm). Identification of tumor margins using reagents emitting in the visible spectrum (400-650 nm), such as fluorescein isothiocyanate (FITC), present challenges considering the presence of auto-fluorescence from tissue and food in the gastrointestinal (GI) tract. To overcome this, Solaris acquires LCTF-based multispectral images, and by applying an automated spectral unmixing algorithm to the data, separates reagent fluorescence from tissue and food auto-fluorescence. The unmixing algorithm uses vertex component analysis to automatically extract the primary pure spectra, and resolves the reagent fluorescent signal using non-negative least squares. For validation, intraoperative in vivo studies were carried out in tumor-bearing rodents injected with FITC-dextran reagent that is primarily residing in malignant tissue 24 hours post injection. In the absence of unmixing, fluorescence from tumors is not distinguishable from that of surrounding tissue. Upon spectral unmixing, the FITC-labeled malignant regions become well defined and detectable. The results of these studies substantiate the multispectral power of Solaris in resolving FITC-based agent signal in deep tumor masses, under ambient and surgical light, and enhancing the ability to surgically resect them.

Paper Details

Date Published: 8 February 2017
PDF: 7 pages
Proc. SPIE 10049, Molecular-Guided Surgery: Molecules, Devices, and Applications III, 100490J (8 February 2017); doi: 10.1117/12.2255669
Show Author Affiliations
Ali Behrooz, PerkinElmer, Inc. (United States)
Kristine O. Vasquez, PerkinElmer, Inc. (United States)
Peter Waterman, PerkinElmer, Inc. (United States)
Jeff Meganck, PerkinElmer, Inc. (United States)
Jeffrey D. Peterson, PerkinElmer, Inc. (United States)
Peter Miller, PerkinElmer, Inc. (United States)
Joshua Kempner, PerkinElmer, Inc. (United States)

Published in SPIE Proceedings Vol. 10049:
Molecular-Guided Surgery: Molecules, Devices, and Applications III
Brian W. Pogue; Sylvain Gioux, Editor(s)

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