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Proceedings Paper

Multiphoton tomography of the human eye
Author(s): Karsten König; Ana Batista; Tobias Hager; Berthold Seitz
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Paper Abstract

Multiphoton tomography (MPT) is a novel label-free clinical imaging method for non-invasive tissue imaging with high spatial (300 nm) and temporal (100 ps) resolutions. In vivo optical histology can be realized due to the nonlinear excitation of endogenous fluorophores and second-harmonic generation (SHG) of collagen. Furthermore, optical metabolic imaging (OMI) is performed by two-photon autofluorescence lifetime imaging (FLIM). So far, applications of the multiphoton tomographs DermaInspect and MPTflex were limited to dermatology. Novel applications include intraoperative brain tumor imaging as well as cornea imaging. In this work we describe two-photon imaging of ex vivo human corneas unsuitable for transplantation. Furthermore, the cross-linking (CXL) process of corneal collagen based on UVA exposure and 0.1 % riboflavin was studied. The pharmacokinetics of the photosensitizer could be detected with high spatial resolution. Interestingly, an increase in the stromal autofluorescence intensity and modifications of the autofluorescence lifetimes were observed in the human corneal samples within a few days following CXL.

Paper Details

Date Published: 21 February 2017
PDF: 8 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006906 (21 February 2017); doi: 10.1117/12.2255151
Show Author Affiliations
Karsten König, Univ. des Saarlandes (Germany)
JenLab GmbH (Germany)
Ana Batista, Univ. des Saarlandes (Germany)
JenLab GmbH (Germany)
Tobias Hager, Univ. des Saarlandes (Germany)
Berthold Seitz, Univ. des Saarlandes (Germany)
Lions Cornea Bank Saar-Lor-Lux (Germany)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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