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Proceedings Paper

Aberrant lipogenesis is a metabolic marker for azole-resistant candida albicans (Conference Presentation)

Paper Abstract

Candida is the single most important cause of fungal bloodstream infections worldwide causing significant mortality as high as 50%. This high mortality rate is, in part, due to the inability to rapidly diagnose and simultaneously initiate an effective antifungal therapy early in the disease process. Current culture-based diagnostics are often slow, requiring several days to complete, and are only 50% sensitive in diagnosing candidemia (Candida bloodstream infection). For every 12 hours of delay in starting correct antifungal therapy, the risk of death for a given patient with candidemia increases by 200%. To address this unmet need, we explored the potential of employing stimulated Raman Scattering (SRS) imaging to diagnose candidemia and probe metabolic differences between resistant and susceptible strain at a single cell level. Metabolism is integral to pathogenicity; microorganism have very short life cycles, and therefore only a few hours are needed to observe a full metabolic cycle. SRS imaging at C-H vibration frequency at 2850 cm-1 revealed a substantial difference in lipogenesis between the susceptible and resistant C. albicans. Treating the C. albicans with fluconazole, an antimicrobial drug that targets ergosterol biosynthesis only affected the lipogenesis in the susceptible strain. Our results show that single-cell metabolic imaging under a SRS microscope can be used for diagnose candidemia and early detection of antimicrobial susceptibility.

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690S (24 April 2017); doi: 10.1117/12.2255078
Show Author Affiliations
Caroline Karanja, Purdue Univ. (United States)
Weili Hong, Purdue Univ. (United States)
Waleed Younis, Purdue Univ. (United States)
Ji-Xin Cheng, Purdue Univ. (United States)
Mohamed Seleem, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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