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Proceedings Paper

Online-FLIM at 10 images per second
Author(s): Wolfgang Becker; Stefan Smietana
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Paper Abstract

We report on a technique that records and displays fluorescence lifetime images at a rate of 10 images per second. Data acquisition is based on multi-dimensional TCSPC in combination with confocal or two-photon laser scanning. The image are calculated from the TCSPC FLIM data via the first moment of the decay data in the pixels of the images. The first-moment technique combines near-ideal photon efficiency with calculation times shorter than the frame times of the commonly used galvanometer scanners. The image rate is thus not slowed down by the lifetime calculation process. Potential applications are clinical FLIM, where suspicious areas can be identified for subsequent high-accuracy imaging, and standard FLIM microscopy, where the technique it helps the user select interesting cells in a large field of view for detailed analysis.

Paper Details

Date Published: 21 February 2017
PDF: 6 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006916 (21 February 2017); doi: 10.1117/12.2254725
Show Author Affiliations
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Stefan Smietana, Becker & Hickl GmbH (Germany)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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