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Proceedings Paper

Optical cell sorting with multiple imaging modalities
Author(s): Andrew Bañas; Caro Carrissemoux; Mark Villangca; Darwin Palima; Jesper Glückstad
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Paper Abstract

Early detection of diseases can save lives. Hence, there is emphasis in sorting rare disease-indicating cells within small dilute quantities such as in the confines of lab-on-a-chip devices. However, before diseased cells can be studied in isolation, it is necessary to identify them against normal healthy cells. With the richness of visual information, a lot of microscopy techniques have been developed and have been crucial in biological studies. To utilize their complementary advantages we adopt both fluorescence and brightfield imaging in our optical cell sorter. Brightfield imaging has the advantage of being non-invasive, thus maintaining cell viability. Fluorescence imaging, on the other hand, takes advantages of the chemical specificity of fluorescence markers and can validate machine vision results from brightfield images. Visually identified cells are sorted using optical manipulation techniques. Scattering forces from beams actuated via efficient phase-only efficient modulation has been adopted. This has lowered the required power for sorting cells to a tenth of our previous approach, and also makes the cell sorter safer for use in clinical settings. With the versatility of dynamically programmable phase spatial light modulators, a plurality of light shaping techniques, including hybrid approaches, can be utilized in cell sorting.

Paper Details

Date Published: 27 February 2017
PDF: 6 pages
Proc. SPIE 10120, Complex Light and Optical Forces XI, 1012018 (27 February 2017); doi: 10.1117/12.2254358
Show Author Affiliations
Andrew Bañas, DTU Fotonik (Denmark)
OptoRobotix ApS (Denmark)
Caro Carrissemoux, Univ. Gent (Belgium)
Mark Villangca, DTU Fotonik (Denmark)
Darwin Palima, DTU Fotonik (Denmark)
Jesper Glückstad, DTU Fotonik (Denmark)
OptoRobotix ApS (Denmark)

Published in SPIE Proceedings Vol. 10120:
Complex Light and Optical Forces XI
David L. Andrews; Enrique J. Galvez; Jesper Glückstad, Editor(s)

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