Share Email Print

Proceedings Paper

Feasibility of using RH795 dye for photoacoustic imaging of neuro-electrical activity
Author(s): Nashaat Rasheed; John R. Cressman; Parag V. Chitnis
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Currently, the most researched noninvasive approach for monitoring neuro-electrical activity involves opticalfluorescence imaging, which suffers from limited imaging penetration. We propose an alternative approach, photoacoustic imaging (PAI) of biopotentials, that relies on transient absorption of light by voltage-sensitive probes and subsequent generation/detection of ultrasound. PAI-based voltage imaging approach can offer the same advantages as the fluorescence imaging in terms of sensitivity and molecular specificity, but it also can significantly extend the imaging depth. In this pilot study we are investigating the feasibility of photoacoustically visualizing biopotentials in rat pheochromocytoma (PC12) cells tagged with voltage-sensitive styrylpyridinium dye, RH795. A change in the intramembrane potential was induced in PC12 cells by adding tetraphenylborate (TPB) to the cell culture. A custommade absorption spectrophotometer was used to verify the change in optical absorption of RH795 dye as a result of TPBinduced electrical fields. Absorption spectra recorded before and after the addition of 100 μM TPB exhibited a wavelength shift of the absorption peak (approximately 510 nm to 550 nm) as well as an increase in the overall magnitude of absorption in the wavelength range of 500-1000 nm. The absorption spectral measurements indicated that RH795 is a good candidate as a voltage-sensitive dye for photoacoustically tracking changes in cell-membrane potential.

Paper Details

Date Published: 8 February 2017
PDF: 6 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 100510X (8 February 2017); doi: 10.1117/12.2253767
Show Author Affiliations
Nashaat Rasheed, George Mason Univ. (United States)
John R. Cressman, George Mason Univ. (United States)
Parag V. Chitnis, George Mason Univ. (United States)

Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?