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Proceedings Paper

Two-photon microscopy measurement of CMRO2 using periarteriolar PO2 gradients (Conference Presentation)
Author(s): Sava Sakadžić; Mohammad A. Yaseen; Rajeshwer S. Jaswal; Emmanuel Roussakis; Anders M. Dale; Richard B. Buxton; Sergei A. Vinogradov; David A. Boas; Anna Devor

Paper Abstract

The cerebral metabolic rate of oxygen (CMRO2) is an essential parameter for evaluating brain function and pathophysiology. Measurements of CMRO2 with high spatio-temporal resolution are critically important for understanding how the brain copes with metabolic and blood perfusion changes associated with various clinical conditions, such as stroke, periinfarct depolarizations, and various microvasculopathies (e.g., Alzheimer’s disease, chronic hypertension). CMRO2 measurements are also important for understanding the physiological underpinnings of functional Magnetic Resonance Imaging signals. However, the currently available approaches for quantifying CMRO2 rely on complex multimodal imaging and mathematical modeling. Here, we introduce a novel method that allows estimation of CMRO2 based on a single measurement modality - two-photon phosphorescence lifetime microscopy (2PLM) imaging of the partial pressure of oxygen (PO2) in cortical tissue. CMRO2 is estimated by fitting the changes of tissue PO2 around cortical penetrating arterioles with the Krogh cylinder model of oxygen diffusion. We measured the baseline CMRO2 in anesthetized rats, and modulated tissue PO2 levels by manipulating the depth of anesthesia. This method has a spatial resolution of approximately 200 μm and it may provide CMRO2 measurements in individual cortical layers or within confined cortical regions such as in ischemic penumbra and the foci of functional activation.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 100510Q (19 April 2017); doi: 10.1117/12.2253690
Show Author Affiliations
Sava Sakadžić, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Mohammad A. Yaseen, Massachusetts General Hospital, Harvard Medical School (United States)
Rajeshwer S. Jaswal, Massachusetts General Hospital, Harvard Medical School (United States)
Emmanuel Roussakis, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Anders M. Dale, Univ. of California, San Diego (United States)
Richard B. Buxton, Univ. of California, San Diego (United States)
Sergei A. Vinogradov, Univ. of Pennsylvania (United States)
David A. Boas, Massachusetts General Hospital, Harvard Medical School (United States)
Anna Devor, Univ. of California, San Diego (United States)
Massachusetts General Hospital, Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

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