Publisher’s Note: This conference presentation, originally published on 4/19/17, was withdrawn per author request.

The immune system is a very complex system that comprises a network of genetic and signaling pathways subtending a network of interacting cells. The location of the cells in a network, along with the gene products they interact with, rules the behavior of the immune system. Therefore, there is a great interest in understanding properly the role of a cell in such networks to increase our knowledge of the immune system response. In order to acquire a better understanding of these processes, cell printing with high spatial resolution emerges as one of the promising approaches to organize cells in two and three-dimensional patterns to enable the study the geometry influence in these interactions. In particular, laser assisted bio-printing techniques using sub-nanosecond laser sources have better characteristics for application in this field, mainly due to its higher spatial resolution, cell viability percentage and process automation. This work presents laser assisted bio-printing of antigen-presenting cells (APCs) in two-dimensional geometries, placing cellular components on a matrix previously generated on demand, permitting to test the molecular interactions between APCs and lymphocytes; as well as the generation of two-dimensional structures designed ad hoc in order to study the mechanisms of mobilization of immune system cells. The use of laser assisted bio-printing, along with APCs and lymphocytes emulate the structure of different niches of the immune system so that we can analyse functional requirement of these interaction.

Date Published: 25 January 2018
PDF: 1 pages
Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII, 100620Q (25 January 2018); doi: 10.1117/12.2253622

Published in SPIE Proceedings Vol. 10062:
Optical Interactions with Tissue and Cells XXVIII
E. Duco Jansen; Hope Thomas Beier, Editor(s)