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Proceedings Paper

Fluorescence lifetime imaging using a single photon avalanche diode array sensor (Conference Presentation)
Author(s): Piotr M. Wargocki; David J. Spence; Ewa M. Goldys; Edoardo Charbon; Claudio E. Bruschini; Ivan Michel Antolović; Samuel Burri

Paper Abstract

Single photon detectors allows us work with the weakest fluorescence signals. Single photon arrays, combined with ps-controlled gating allow us to create image maps of fluorescence lifetimes, which can be used for in-vivo discrimination of tissue activity. Here we present fluorescence lifetime imaging using the ‘SwissSPAD’ sensor, a 512-by-128-pixel array of gated single photon detectors, fabricated in a standard high-voltage 0.35 μm CMOS process. We present a protocol for spatially resolved lifetime measurements where the lifetime can be retrieved for each pixel. We demonstrate the system by imaging patterns of Fluorescein and Rhodamine B on test slides, as well as measuring mixed samples to retrieve both components of the decay lifetime. The single photon sensitivity of the sensor creates a valuable instrument to perform live cell or live animal (in vivo) measurements of the weak autofluorescent signals, for example distinguishing unlabelled free and bound NADH. Our ultimate goal is to create a real time fluorescence lifetime imaging system, possibly integrated into augmented reality goggles, which could allow immediate discrimination of in vivo tissues.

Paper Details

Date Published: 19 June 2017
PDF: 1 pages
Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100680W (19 June 2017); doi: 10.1117/12.2253579
Show Author Affiliations
Piotr M. Wargocki, Macquarie Univ. (Australia)
David J. Spence, Macquarie Univ. (Australia)
Ewa M. Goldys, Macquarie Univ. (Australia)
Edoardo Charbon, Technische Univ. Delft (Netherlands)
Claudio E. Bruschini, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Ivan Michel Antolović, Technische Univ. Delft (Netherlands)
Samuel Burri, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 10068:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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