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Proceedings Paper

Toward investigating changes in cell mechanoelastic properties in response to nanosecond pulsed electric fields
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Paper Abstract

Nanosecond electric pulses (nsEPs) are known to cause a variety of effects on mammalian cells, ranging from destabilization of cell membranes to changes in cytoskeleton and elastic moduli. Measurement of a cells mechanoelastic properties have previously been limited to only invasive and destructive techniques such as atomic force microscopy or application of optical tweezers. However, due to recent advances, Brillouin spectroscopy has now become viable as a non-contact, non-invasive method for measuring these properties in cells and other materials. Here, we present progress toward applying Brillouin spectroscopy using a unique microscopy system for measuring changes in CHO-K1 cells when exposed to nsEPs of 600ns pulse duration with intensity of 50kV/cm. Successful measurement of mechanoelastic changes in these cells will demonstrate Brillouin spectroscopy as a viable method for measuring changes in elastic properties of other cells and living organisms.

Paper Details

Date Published: 22 February 2017
PDF: 6 pages
Proc. SPIE 10066, Energy-based Treatment of Tissue and Assessment IX, 1006604 (22 February 2017); doi: 10.1117/12.2253451
Show Author Affiliations
Zachary Coker, Texas A&M Univ. (United States)
Air Force Research Lab. (United States)
Maria Troyanova-Wood, Texas A&M Univ. (United States)
Andrew Traverso, Texas A&M Univ. (United States)
Zhaokai Meng, Texas A&M Univ. (United States)
Charles Ballmann, Texas A&M Univ. (United States)
Georgi Petrov, Texas A&M Univ. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Vladislav Yakovlev, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 10066:
Energy-based Treatment of Tissue and Assessment IX
Thomas P. Ryan, Editor(s)

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