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Proceedings Paper

Referencing techniques for high-speed confocal fluorescence lifetime imaging microscopy (FLIM) based on analog mean-delay (AMD) method
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Paper Abstract

Analog mean-delay (AMD) method is a new powerful alternative method in determining the lifetime of a fluorescence molecule for high-speed confocal fluorescence lifetime imaging microscopy (FLIM). Even though the photon economy and the lifetime precision of the AMD method are proven to be as good as the state-of-the-art time-correlated single photon counting (TC-SPC) method, there have been some speculations and concerns about the accuracy of this method. In the AMD method, the temporal waveform of an emitted fluorescence signal is directly recorded with a slow digitizer whose bandwidth is much lower than the temporal resolution of lifetime to be measured. We found that the drifts and the fluctuations of the absolute zero position in a measured temporal waveform are the major problems in the AMD method. As a referencing technique, we already proposed dual-channel waveform measurement scheme that may suppress these errors. In this study, we have demonstrated real-time confocal AMD-FLIM system with dual-channel waveform measurement technique.

Paper Details

Date Published: 16 February 2017
PDF: 6 pages
Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681Z (16 February 2017);
Show Author Affiliations
Byungyeon Kim, Osong Medical Innovation Foundation (Korea, Republic of)
Minsuk Lee, Osong Medical Innovation Foundation (Korea, Republic of)
Byungjun Park, Osong Medical Innovation Foundation (Korea, Republic of)
Seungrag Lee, Osong Medical Innovation Foundation (Korea, Republic of)
Youngjae Won, Osong Medical Innovation Foundation (Korea, Republic of)


Published in SPIE Proceedings Vol. 10068:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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