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Proceedings Paper

Multiplexed lasing in tissues
Author(s): Yu-Cheng Chen; Qiushu Chen; Xudong Fan
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Paper Abstract

Biolasers are an emerging technology for next generation biochemical detection and clinical applications. Progress has recently been made to achieve lasing from biomolecules and single living cells. Tissues, which consist of cells embedded in extracellular matrix, mimic more closely the actual complex biological environment in a living body and therefore are of more practical significance. Here, we developed a highly versatile tissue laser platform, in which tissues stained with fluorophores are sandwiched in a high-Q Fabry-Pérot microcavity. Distinct lasing emissions from muscle and adipose tissues stained respectively with fluorescein isothiocyanate (FITC) and boron-dipyrromethene (BODIPY), and hybrid muscle/adipose tissue with dual-staining were achieved with a threshold of only ~10 μJ/mm2. Additionally, we investigated how tissue structure/geometry, tissue thickness, and staining dye concentration affect the tissue laser. It is further found that, despite large fluorescence spectral overlap between FITC and BODIPY in tissues, their lasing emissions could be clearly distinguished and controlled due to their narrow lasing bands and different lasing thresholds, thus enabling highly multiplexed detection. Our tissue laser platform can be broadly applicable to various types of tissues/diseases. It provides a new tool for a wide range of biological and biomedical applications, such as diagnostics/screening of tissues and identification/monitoring of biological transformations in tissue engineering.

Paper Details

Date Published: 22 February 2017
PDF: 11 pages
Proc. SPIE 10075, Biophysics, Biology and Biophotonics II: the Crossroads, 1007506 (22 February 2017); doi: 10.1117/12.2253273
Show Author Affiliations
Yu-Cheng Chen, Univ. of Michigan (United States)
Qiushu Chen, Univ. of Michigan (United States)
Xudong Fan, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 10075:
Biophysics, Biology and Biophotonics II: the Crossroads
Adam Wax; Vadim Backman, Editor(s)

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