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Proceedings Paper

Design and implementation of a dual-wavelength intrinsic fluorescence camera system
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Paper Abstract

Intrinsic UV fluorescence imaging is a technique that permits the observation of spatial differences in emitted fluorescence. It relies on the fluorescence produced by the innate fluorophores in the sample, and thus can be used for marker-less in-vivo assessment of tissue. It has been studied as a tool for the study of the skin, specifically for the classification of lesions, the delimitation of lesion borders and the study of wound healing, among others. In its most basic setup, a sample is excited with a narrow-band UV light source and the resulting fluorescence is imaged with a UV sensitive camera filtered to the emission wavelength of interest. By carefully selecting the excitation/emission pair, we can observe changes in fluorescence associated with physiological processes. One of the main drawbacks of this simple setup is the inability to observe more than a single excitation/emission pair at the same time, as some phenomena are better studied when two or more different pairs are studied simultaneously. In this work, we describe the design and the hardware and software implementation of a dual wavelength portable UV fluorescence imaging system. Its main components are an UV camera, a dual wavelength UV LED illuminator (295 and 345 nm) and two different emission filters (345 and 390 nm) that can be swapped by a mechanical filter wheel. The system is operated using a laptop computer and custom software that performs basic pre-processing to improve the image. The system was designed to allow us to image fluorescent peaks of tryptophan and collagen cross links in order to study wound healing progression.

Paper Details

Date Published: 14 March 2017
PDF: 6 pages
Proc. SPIE 10056, Design and Quality for Biomedical Technologies X, 100560U (14 March 2017); doi: 10.1117/12.2253063
Show Author Affiliations
Antonio Ortega-Martinez, Wellman Ctr. for Photomedicine, Harvard Medical School (United States)
Joseph J. Musacchia, Wellman Ctr. for Photomedicine, Harvard Medical School (United States)
Enoch Gutierrez-Herrera, Univ. Nacional Autónoma de México (Mexico)
Ying Wang, Wellman Ctr. for Photomedicine, Harvard Medical School (United States)
Walfre Franco, Wellman Ctr. for Photomedicine, Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 10056:
Design and Quality for Biomedical Technologies X
Ramesh Raghavachari; Rongguang Liang, Editor(s)

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