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Proceedings Paper

Low-cost production and sealing procedure of mechanical parts of a versatile 3D-printed perfusion chamber for digital holographic microscopy of primary neurons in culture
Author(s): Erik Bélanger; Sébastien A. Lévesque; Gabriel Anctil; Anne-Sophie Poulin-Girard; Pierre Marquet M.D.
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Paper Abstract

We have developed a prototype of a low-cost and versatile 3D-printed perfusion chamber for digital holographic microscopy (DHM) of primary neurons in culture. The imaging chamber is 3D-printed in biocompatible plastic. It is easily convertible between a closed configuration, for refractive index - cellular thickness decoupling, and an open configuration, for electrophysiology. In the closed arrangement, the imaging volume is small, allowing a rapid laminar flow with a fast turnover for an optimal implementation of the decoupling procedure. This paper highlights especially the challenges faced while designing and prototyping the 3D-printed closed perfusion chamber with a small imaging volume for DHM. As all 3D-printed mechanical parts were initially leaking because of internal porosities, we developed a simple sealing protocol using acetone vapors to smooth surfaces. Using this protocol, almost all mechanical parts were successfully sealed. Therefore, the production process of the actual prototype, i.e. the 3D printing and the sealing method, is satisfactory for our target application in the field of microfluidics.

Paper Details

Date Published: 21 February 2017
PDF: 7 pages
Proc. SPIE 10074, Quantitative Phase Imaging III, 100741S (21 February 2017); doi: 10.1117/12.2253036
Show Author Affiliations
Erik Bélanger, Institut Univ. en Santé Mentale de Québec, Univ. Laval (Canada)
Sébastien A. Lévesque, Institut Univ. en Santé Mentale de Québec, Univ. Laval (Canada)
Gabriel Anctil, Institut Univ. en Santé Mentale de Québec, Univ. Laval (Canada)
Anne-Sophie Poulin-Girard, Institut Univ. en Santé Mentale de Québec, Univ. Laval (Canada)
Pierre Marquet M.D., Institut Univ. en Santé Mentale de Québec, Univ. Laval (Canada)


Published in SPIE Proceedings Vol. 10074:
Quantitative Phase Imaging III
Gabriel Popescu; YongKeun Park, Editor(s)

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