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Proceedings Paper

Evaluation of structural remodeling of the atria with OCT in a chronic rat model of myocardial infarction (Conference Presentation)
Author(s): Melissa M. Eberle; Stephanie Thorn; Lawerence Young; Daniel Pfau; Jeffrey Madwed; Kersten Small; Michael Kilmas; Michael A. Choma; Albert J. Sinusas

Paper Abstract

Atrial fibrillation (AF) occurs following myocardial infarction (MI) and is associated with left ventricular dysfunction, which promotes the development of atrial remodeling and permanent atrial fibrosis. The purpose of this study was determining the effects of MI on left atrial (LA) remodeling with and without therapy with an angiotensin converting enzyme inhibition (ACEi) utilizing optical coherence tomography (OCT). As the composition of the myocardial tissue changes during LA remodeling the optical attenuation of the light will also change providing a metric to quantify the structural remodeling process. Lewis rats (240-275 g) underwent either surgical ligation of left coronary artery creating chronic MI, or SHAM surgery. 13 weeks post-surgery, ex vivo OCT imaging was performed of the LA appendage. Depth-resolved, attenuation coefficient volumes were calculated and the resulting atrial wall attenuation values were analyzed for four experimental groups: SHAM, SHAM with ACEi, MI no ACEi, and MI with ACEi. Quantification of tissue attenuation was performed and shown to significantly increase with MI in association with increases in collagen as verified with corresponding histological sectioning. Fractal analysis of the LA wall trabeculation patterns, 100 µm below the surface, was performed to quantify wall thickening associated with LA remodeling. A significant increase in fractal dimension was determined post MI compared to SHAM corresponding to a loss of the trabeculation pattern and wall thickening. The results from this study demonstrate OCT as an imaging technique capable of investigate LA remodeling with high resolution and label-free optical contrast processing.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10042, Diagnostic and Therapeutic Applications of Light in Cardiology, 1004206 (19 April 2017); doi: 10.1117/12.2252954
Show Author Affiliations
Melissa M. Eberle, Yale Univ. (United States)
Stephanie Thorn, Yale Univ. (United States)
Lawerence Young, Yale Univ. (United States)
Daniel Pfau, Yale Univ. (United States)
Jeffrey Madwed, Merck Research Laboratory (United States)
Kersten Small, Merck Research Labs. (United States)
Michael Kilmas, Merck Research Labs. (United States)
Michael A. Choma, Yale School of Medicine (United States)
Albert J. Sinusas, Yale Univ. (United States)

Published in SPIE Proceedings Vol. 10042:
Diagnostic and Therapeutic Applications of Light in Cardiology
Guillermo J. Tearney M.D.; Kenton W. Gregory M.D.; Laura Marcu, Editor(s)

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