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Proceedings Paper

Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy
Author(s): Junwei Min; Baoli Yao; Steffi Ketelhut; Björn Kemper
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Paper Abstract

The modular combination of optical microscopes with digital holographic microscopy (DHM) has been proven to be a powerful tool for quantitative live cell imaging. The introduction of condenser and different microscope objectives (MO) simplifies the usage of the technique and makes it easier to measure different kinds of specimens with different magnifications. However, the high flexibility of illumination and imaging also causes variable phase aberrations that need to be eliminated for high resolution quantitative phase imaging. The existent phase aberrations compensation methods either require add additional elements into the reference arm or need specimen free reference areas or separate reference holograms to build up suitable digital phase masks. These inherent requirements make them unpractical for usage with highly variable illumination and imaging systems and prevent on-line monitoring of living cells. In this paper, we present a simple numerical method for phase aberration compensation based on the analysis of holograms in spatial frequency domain with capabilities for on-line quantitative phase imaging. From a single shot off-axis hologram, the whole phase aberration can be eliminated automatically without numerical fitting or pre-knowledge of the setup. The capabilities and robustness for quantitative phase imaging of living cancer cells are demonstrated.

Paper Details

Date Published: 17 February 2017
PDF: 6 pages
Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 100700A (17 February 2017); doi: 10.1117/12.2252883
Show Author Affiliations
Junwei Min, Westfälische Wilhelms-Univ. Münster (Germany)
Xi'an Institute of Optics and Precision Mechanics (China)
Baoli Yao, Xi'an Institute of Optics and Precision Mechanics (China)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 10070:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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