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Proceedings Paper

Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup
Author(s): Nir A. Turko; Michael Isbach; Steffi Ketelhut; Burkhard Greve; Jürgen Schnekenburger; Natan T. Shaked; Björn Kemper
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Paper Abstract

We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

Paper Details

Date Published: 21 February 2017
PDF: 7 pages
Proc. SPIE 10074, Quantitative Phase Imaging III, 100740Q (21 February 2017); doi: 10.1117/12.2252873
Show Author Affiliations
Nir A. Turko, Tel Aviv Univ. (Israel)
Michael Isbach, Westfälische Wilhelms-Univ. Münster (Germany)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)
Burkhard Greve, Westfälische Wilhelms-Univ. Münster (Germany)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ. Münster (Germany)
Natan T. Shaked, Tel Aviv Univ. (Israel)
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 10074:
Quantitative Phase Imaging III
Gabriel Popescu; YongKeun Park, Editor(s)

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