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Proceedings Paper

Therapeutic drug monitoring of flucytosine in serum using a SERS-active membrane system
Author(s): Adam G. Berger; Ian M. White
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Paper Abstract

A need exists for near real-time therapeutic drug monitoring (TDM), in particular for antibiotics and antifungals in patient samples at the point-of-care. To truly fit the point-of-care need, techniques must be rapid and easy to use. Here we report a membrane system utilizing inkjet-fabricated surface enhanced Raman spectroscopy (SERS) sensors that allows sensitive and specific analysis despite the elimination of sophisticated chromatography equipment, expensive analytical instruments, and other systems relegated to the central lab. We utilize inkjet-fabricated paper SERS sensors as substrates for 5FC detection; the use of paper-based SERS substrates leverages the natural wicking ability and filtering properties of microporous membranes. We investigate the use of microporous membranes in the vertical flow assay to allow separation of the flucytosine from whole blood. The passive vertical flow assay serves as a valuable method for physical separation of target analytes from complex biological matrices. This work further establishes a platform for easy, sensitive, and specific TDM of 5FC from whole blood.

Paper Details

Date Published: 23 February 2017
PDF: 7 pages
Proc. SPIE 10081, Frontiers in Biological Detection: From Nanosensors to Systems IX, 1008104 (23 February 2017); doi: 10.1117/12.2252678
Show Author Affiliations
Adam G. Berger, Univ. of Maryland, College Park (United States)
Ian M. White, Univ. of Maryland, College Park (United States)

Published in SPIE Proceedings Vol. 10081:
Frontiers in Biological Detection: From Nanosensors to Systems IX
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

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