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Ex-vivo UV autofluorescence imaging and fluorescence spectroscopy of atherosclerotic pathology in human aorta
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Paper Abstract

The aim of our study was to identify fluorescence excitation-emission pairs correlated with atherosclerotic pathology in ex-vivo human aorta. Wide-field images of atherosclerotic human aorta were captured using UV and visible excitation and emission wavelength pairs of several known fluorophores to investigate correspondence with gross pathologic features. Fluorescence spectroscopy and histology were performed on 21 aortic samples. A matrix of Pearson correlation coefficients were determined for the relationship between relevant histologic features and the intensity of emission for 427 wavelength pairs. A multiple linear regression analysis indicated that elastin (370/460 nm) and tryptophan (290/340 nm) fluorescence predicted 58% of the variance in intima thickness (R-squared = 0.588, F(2,18) = 12.8, p=.0003), and 48% of the variance in media thickness (R-squared = 0.483, F(2,18) = 8.42, p=.002), suggesting that endogenous fluorescence intensity at these wavelengths can be utilized for improved pathologic characterization of atherosclerotic plaques.

Paper Details

Date Published: 8 February 2017
PDF: 12 pages
Proc. SPIE 10042, Diagnostic and Therapeutic Applications of Light in Cardiology, 100420Q (8 February 2017); doi: 10.1117/12.2252590
Show Author Affiliations
William Lewis, Wellman Ctr. for Photomedicine (United States)
Maura Williams, Wellman Ctr. for Photomedicine (United States)
Walfre Franco, Wellman Ctr. for Photomedicine (United States)

Published in SPIE Proceedings Vol. 10042:
Diagnostic and Therapeutic Applications of Light in Cardiology
Guillermo J. Tearney M.D.; Kenton W. Gregory M.D.; Laura Marcu, Editor(s)

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