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Proceedings Paper

Wavefront coding for fast, high-resolution light-sheet microscopy (Conference Presentation)

Paper Abstract

Some biological experiments demand the observation of dynamics processes in 3D with high spatiotemporal resolution. The use of wavefront coding to extend the depth-of-field (DOF) of the collection arm of a light-sheet microscope is an interesting alternative for fast 3D imaging. Under this scheme, the 3D features of the sample are captured at high volumetric rates while the light sheet is swept rapidly within the extended DOF. The DOF is extended by coding the pupil function of the imaging lens by using a custom-designed phase mask. A posterior restoration step is required to decode the information of the captured images based on the applied phase mask [1]. This hybrid optical-digital approach is known as wavefront coding (WFC). Previously, we have demonstrated this method for performing fast 3D imaging of biological samples at medium resolution [2]. In this work, we present the extension of this approach for high-resolution microscopes. Under these conditions, the effective DOF of a standard high NA objective is of a few micrometers. Here we demonstrate that by the use of WFC, we can extend the DOF more than one order of magnitude keeping the high-resolution imaging. This is demonstrated for two designed phase masks using Zebrafish and C. elegans samples. [1] Olarte, O.E., Andilla, J., Artigas, D., and Loza-Alvarez, P., “Decoupled Illumination-Detection Microscopy. Selected Optics in Year 2105,” in Optics and Photonics news 26, p. 41 (2015). [2] Olarte, O.E., Andilla, J., Artigas, D., and Loza-Alvarez, P., “Decoupled illumination detection in light sheet microscopy for fast volumetric imaging,” Optica 2(8), 702 (2015).

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 1007307 (24 April 2017); doi: 10.1117/12.2252574
Show Author Affiliations
Omar E. Olarte, ICFO - Institut de Ciències Fotòniques (Spain)
Jacob Licea-Rodriguez, ICFO - Institut de Ciències Fotòniques (Spain)
Pablo Loza-Alvarez, ICFO - Institut de Ciències Fotòniques (Spain)

Published in SPIE Proceedings Vol. 10073:
Adaptive Optics and Wavefront Control for Biological Systems III
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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