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Proceedings Paper

Epi-direction detected multimodal imaging of an unstained mouse retina with a Yb-fiber laser
Author(s): Gabrielle A. Murashova; Christopher A. Mancuso; Sanae Sakami; Krzysztof Palczewski; Grazyna Palczewska; Marcos Dantus
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Paper Abstract

In this work, we present all epi-direction detected images of an unstained mouse retina using multiphoton microscopy with a sub-50 fs Yb-fiber laser centered at 1.07 μm. This wavelength regime is particularly important as the fundamental wavelength is transparent to the eye and the higher harmonics are above the DNA-damaging UV wavelengths. We present a characterization of the multimodal signals emitted from all the retinal layers, as well as from the choroid and the sclera. By characterizing native multiphoton signals from the retina, we move closer to having Yb-fiber considered for in vivo diagnosis of retinal disease through multiphoton microscopy as well as for corrective therapies.

Paper Details

Date Published: 2 March 2017
PDF: 5 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692K (2 March 2017); doi: 10.1117/12.2252457
Show Author Affiliations
Gabrielle A. Murashova, Michigan State Univ. (United States)
Christopher A. Mancuso, Michigan State Univ. (United States)
Sanae Sakami, Case Western Reserve Univ. (United States)
Krzysztof Palczewski, Case Western Reserve Univ. (United States)
Grazyna Palczewska, Polgenix Inc. (United States)
Marcos Dantus, Michigan State Univ. (United States)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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