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Proceedings Paper

Longitudinal visualization of vascular occlusion, reperfusion, and remodeling in a zebrafish model of retinal vascular leakage using OCT angiography
Author(s): Kathleen Spitz; Ivan Bozic; Vineet Desai; Gopikrishna M. Rao; Lana M. Pollock; Bela Anand-Apte; Yuankai K. Tao
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Paper Abstract

Diabetic retinopathy (DR) and age-related macular degeneration (AMD) are two of the leading causes of blindness and visual impairment in the world. Neovascularization results in severe vision loss in DR and AMD and, thus, there is an unmet need to identify mechanisms of pathogenesis and novel anti-angiogenic therapies. Zebrafish is a leading model organism for studying human disease pathogenesis, and the highly conserved drug activity between zebrafish and humans and their ability to readily absorb small molecules dissolved in water has benefited pharmaceutical discovery. Here, we use optical coherence tomography (OCT) and OCT angiography (OCT-A) to perform noninvasive, in vivo retinal imaging in a zebrafish model of vascular leakage. Zebrafish were treated with diethylaminobenzaldehyde (DEAB) to induce vascular leakage and imaged with OCT and OCT-A at six time points over two weeks: baseline one day before treatment and one, three, six, eight, and ten days post treatment. Longitudinal functional imaging showed significant vascular response immediately after DEAB treatment. Observed vascular changes included partial or complete vascular occlusion immediately after treatment and reperfusion during a two-week period. Increased vascular tortuosity several days post treatment indicated remodeling, and bifurcations and collateral vessel formation were also observed. In addition, significant treatment response variabilities were observed in the contralateral eye of the same animal. Anatomical and functional normalization was observed in most animals by ten days post treatment. These preliminary results motivate potential applications of OCT-A as a tool for studying pathogenesis and therapeutic screening in zebrafish models of retinal vascular disease.

Paper Details

Date Published: 8 February 2017
PDF: 4 pages
Proc. SPIE 10045, Ophthalmic Technologies XXVII, 1004525 (8 February 2017); doi: 10.1117/12.2252418
Show Author Affiliations
Kathleen Spitz, Vanderbilt Univ. (United States)
Ivan Bozic, Vanderbilt Univ. (United States)
Vineet Desai, Vanderbilt Univ. (United States)
Gopikrishna M. Rao, Vanderbilt Univ. (United States)
Lana M. Pollock, The Cleveland Clinic (United States)
Bela Anand-Apte, The Cleveland Clinic (United States)
Yuankai K. Tao, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 10045:
Ophthalmic Technologies XXVII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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